Virtual Microscope is a Java application that supports interactive viewing of high-resolution, multi-dimensional image datasets from various microscopes. We currently support data from a Philips Environmental Scanning Electron Microscope (ESEM), and a Fluorescence Light Microscope. The interface provides a simulation of an actual microscope interface. Controls common to all datasets include brightness, magnification (usually up to 1800x), navigation, measurement, and annotation. SEM samples include a contrast control, and some also include a focus control. The navigation and magnification controls allow the user to explore any point of interest on the sample. This differs from other virtual microscopes that have been produced which provide only a few 'hot spots' that can be magnified and explored.